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- Recombinant DNA technologies -
Recombinant cells can be prepared following DNA cloning step through transformation or transfection.
-> Transformed cells will be amplified by liquid culture. (This cycling process is performed for Directed Evolution.)
-> More effective protein candidates were chosen via Screening and assay step.
-> The best positive hit will be used for production of bio-contents.
-> High-level expression in E. coli, yeast, insect, and mammallial cells.